Journal: International journal of molecular sciences

Article Title: IL-6 Does Not Influence the Expression of SLC41A1 and Other Mg-Homeostatic Factors.

doi: 10.3390/ijms252413274

Figure Lengend Snippet: Figure 4. Treatment of HEK-Blue™IL-6, HepG2, U-266, and PANC-1 cell lines with 50 ng/mL hIL-6 for 30 min or 3 h triggered STAT3 phosphorylation at Y705. In the case of HepG2 and U-266, S727

Article Snippet: In our experiments, we used four different human cell lines: IL-6 reporter line HEKBlueTM IL-6 Cells (Invitrogen, Carlsbad, CA, USA), hepatoblastoma HepG2, multiple myeloma U-266, and pancreas carcinoma PANC-1 (all three DSMZ, Braunschweig, Germany), cultured at 37 ◦C in a humidified atmosphere of 5% CO2 and maintained in the culture media as recommended by the supplier and supplemented with 10% heat-inactivated fetal bovine serum and 1% Pen-Strep (HEK-BlueTM IL-6 Cells-DMEM 4.5 g/L glucose, HEK-BlueTM Selection (InvivoGen, San Diego, CA, USA), NormocinTM (InvivoGen, USA); HepG2-DMEM 1 g/L glucose; U-266-RPMI 1640, 1 mmol/L sodium pyruvate, 4.5 g/L glucose; PANC-1-DMEM 4.5 g/L glucose).

Techniques: Phospho-proteomics

Journal: International journal of molecular sciences

Article Title: IL-6 Does Not Influence the Expression of SLC41A1 and Other Mg-Homeostatic Factors.

doi: 10.3390/ijms252413274

Figure Lengend Snippet: Figure 5. Relative expression ratios of eight analyzed magnesiotropic genes normalized to the expression rate of three reference genes (ACTB, GAPDH, YWHAZ). Cells were treated with hIL-6 at a concentration of 1 or 50 ng/mL for 1 or 24 h. Experiments were carried out with three cell lines, namely HepG2 (a), U-266 (b), and PANC-1 (c), in three independent replicates. The means, excluding outliers, are presented together with the standard deviation.

Article Snippet: In our experiments, we used four different human cell lines: IL-6 reporter line HEKBlueTM IL-6 Cells (Invitrogen, Carlsbad, CA, USA), hepatoblastoma HepG2, multiple myeloma U-266, and pancreas carcinoma PANC-1 (all three DSMZ, Braunschweig, Germany), cultured at 37 ◦C in a humidified atmosphere of 5% CO2 and maintained in the culture media as recommended by the supplier and supplemented with 10% heat-inactivated fetal bovine serum and 1% Pen-Strep (HEK-BlueTM IL-6 Cells-DMEM 4.5 g/L glucose, HEK-BlueTM Selection (InvivoGen, San Diego, CA, USA), NormocinTM (InvivoGen, USA); HepG2-DMEM 1 g/L glucose; U-266-RPMI 1640, 1 mmol/L sodium pyruvate, 4.5 g/L glucose; PANC-1-DMEM 4.5 g/L glucose).

Techniques: Expressing, Concentration Assay, Standard Deviation